猪瘟病毒促进细胞自噬并利于病毒增殖

doi:10.11843/j.issn.0366-6964.2014.09.014

Abstract

Abstract:

Autophagy plays an important role in virus proliferation and release while there have no report about the relationship of classical swine fever virus (CSFV) and host cell autophagy.This study was undertaken to elucidate the impact of CSFV Shimen strain on host cell (ST) autophagy and the effect of autophagy to virus proliferation.The transmission electron microscopy (sem)，LC3 dots，biomarkers of autophagy，were adopt to get a preliminary determination.The protein expression of LC3 and P62，as well as conversion of LC3Ⅰto LC3 Ⅱwere semi-quantified by Western blot，the results suggested that the CSFV promotes cell autophagy.Autophagy promoter rapamycin，inhibitors 3-MA，chloroquine(CQ)，fusion blocker of autophagosome and lysosomes，were used to regulate the autophagy，as well as autophagy associated gene (ATG) Beclin 1 and LC3 protein were interfered to investigate the effect of autophagy on virus proliferation.The results showed that virus infection promotes cell autophagy and form complete autophagy flux，which facilitates virus proliferation.This research provides new information to the relationship of CSFV and host cell.

CLC Number:

KANG Kai，LIN Zhi，GAO Hai-hui，ZHANG Cheng-cheng，LI He-lin，LIANG Wu-long，WANG Jing，CAO Zhi，ZHANG Yan-ming. Classical Swine Fever Virus Promotes Cell Autophagy which Facilitates Virus Proliferation[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(9): 1481-1487.

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https://www.xmsyxb.com/EN/Y2014/V45/I9/1481

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Classical Swine Fever Virus Promotes Cell Autophagy which Facilitates Virus Proliferation

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